GSM1904942: B171_DMEM_0.5_BR2_2; Escherichia coli B171; RNA-Seq - SRA

SRX1322480: GSM1904942: B171_DMEM_0.5_BR2_2; Escherichia coli B171; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 23.5M spots, 4.7G bases, 3.3Gb downloads

Submitted by: Gene Expression Omnibus (GEO)

Study: Comparative transcriptomics of the virulence regulons of enteropathogenic Escherichia coli from diverse phylogenomic lineages

PRJNA298339 • SRP064662 • All experiments • All runs

show Abstracthide Abstract

Global transcriptional analysis of eleven EPEC strains, including the most frequently studied strains used in laboratory studies investigating EPEC virulence mechanisms. Overall design: RNA sequencing was performed on the four EPEC prototype strains during growth in laboratory media and conditions that have been previously demonstrated to induce expression of EPEC virulence factors.

Sample: B171_DMEM_0.5_BR2_2

SAMN04158445 • SRS1109916 • All experiments • All runs

Organism: Escherichia coli B171

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: RNA was isolated from cells grown in LB or DMEM using the Ribopure Bacteria kit (Ambion) and contaminating DNA was removed using the Ribopure Dnase I and also the Turbo DNA-free kit (Ambion). RNA libraries were prepared for sequencing using standard Illumina protocols

Experiment attributes:

GEO Accession: GSM1904942

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 23.5M spots, 4.7G bases, 3.3Gb

Run	# of Spots	# of Bases	Size	Published
SRR2601728	23,498,840	4.7G	3.3Gb	2017-05-10

ID:: 1887157

SRA

Sequence Read Archive

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